Bacterial transfer of large functional genomic DNA into human cells

  title={Bacterial transfer of large functional genomic DNA into human cells},
  author={A. Laner and Sylvie Goussard and Anabela S Ramalho and Tobias Schwarz and Margarida D Amaral and Patrice Courvalin and Dirk Schindelhauer and Catherine Grillot-Courvalin},
  journal={Gene Therapy},
Efficient transfer of chromosome-based vectors into mammalian cells is difficult, mostly due to their large size. Using a genetically engineered invasive Escherichia coli vector, alpha satellite DNA cloned in P1-based artificial chromosome was stably delivered into the HT1080 cell line and efficiently generated human artificial chromosomes de novo. Similarly, a large genomic cystic fibrosis transmembrane conductance regulator (CFTR) construct of 160 kb containing a portion of the CFTR gene was… CONTINUE READING
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