Bacterial lipases: an overview of production, purification and biochemical properties

@article{Gupta2004BacterialLA,
  title={Bacterial lipases: an overview of production, purification and biochemical properties},
  author={R. Gupta and N. Gupta and P. Rathi},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={64},
  pages={763-781}
}
Abstract Lipases, triacylglycerol hydrolases, are an important group of biotechnologically relevant enzymes and they find immense applications in food, dairy, detergent and pharmaceutical industries. Lipases are by and large produced from microbes and specifically bacterial lipases play a vital role in commercial ventures. Some important lipase-producing bacterial genera include Bacillus, Pseudomonas and Burkholderia. Lipases are generally produced on lipidic carbon, such as oils, fatty acids… Expand
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Commercialization of lipase production is a prime area of interest for microbiologists, process engineers and biochemists as microbes, especially fungi and bacteria, are the tools of choice for commercial production. Expand
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Direct evolution based on error prone PCR, the enantioselectivity of the hydrolysis of a chiral ester, catalyzed by the lipase from P. aeruginosa, can be increased from ee 2 to ee 81% in just four mutagenesis cycles. Expand
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Three-dimensional structures of bacterial lipases were solved to understand the catalytic mechanism of lipase reactions and will enable researchers to tailor new lipases for biotechnological applications. Expand
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TLDR
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TLDR
The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia (PcL) in the absence of a bound inhibitor using X-ray crystallography suggests that this enzyme shares the same mechanisms of catalysis and interfacial activation as other lipases. Expand
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TLDR
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TLDR
The X-ray studies of pure lipases will enable the establishment of the structure-function relationships and contribute for a better understanding of the kinetic mechanisms of lipase action on hydrolysis, synthesis and group exchange of esters. Expand
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