Bacterial initiators form dynamic filaments on single-stranded DNA monomer by monomer

@inproceedings{Cheng2015BacterialIF,
  title={Bacterial initiators form dynamic filaments on single-stranded DNA monomer by monomer},
  author={Hsin-Mei Cheng and Philip Gr{\"o}ger and Andreas Hartmann and Michael Schlierf},
  booktitle={Nucleic acids research},
  year={2015}
}
DNA replication initiation is mediated across all domains of life by initiator proteins oligomerizing at replication origins. Recently, it was shown that initiators can directly bind single-stranded DNA (ssDNA) and thus might enhance origin melting. In this study, we used single-molecule fluorescence assays to probe the ssDNA binding mechanism of the replication initiator DnaA. Our experiments revealed that DnaA forms a dynamic filament on ssDNA in 3' to 5' directionality in the presence of ATP… CONTINUE READING

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