Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size.

  title={Bacterial fitness and plasmid loss: the importance of culture conditions and plasmid size.},
  author={M. A. K. Smith and Michael J Bidochka},
  journal={Canadian journal of microbiology},
  volume={44 4},
Several pBluescript-derived plasmids of various sizes were constructed to study the effects of multicopy plasmid size on bacterial fitness and plasmid loss. Transformed and untransformed bacterial clones were grown in media with or without ampicillin. Bacterial fitness (measured by growth rate), plasmid presence or absence, and plasmid copy number were assessed during successive subculturings. In selective media (minimal medium or Luria Broth plus ampicillin), the clone transformed with the… 

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