Bacterial expression of immunoglobulin fragments.

  title={Bacterial expression of immunoglobulin fragments.},
  author={Arne Skerra},
  journal={Current opinion in immunology},
  volume={5 2},
  • Arne Skerra
  • Published 1993 in Current opinion in immunology
The expression of Ig fragments in Escherichia coli permits rapid access to engineered molecules with antigen-binding properties. While the expression in a functional state by secretion to the periplasm is the standard method for the production of Fv and Fab fragments, single chain Fv fragments are mainly produced by refolding from insoluble aggregates. Although all of these Ig fragments serve as valuable aids in the study of antigen binding, their different biochemical properties must be… CONTINUE READING