Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein.

@article{Liu2001BacterialEO,
  title={Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein.},
  author={Qiang Liu and Philip Willson and S Attoh-Poku and Lorne A. Babiuk},
  journal={Protein expression and purification},
  year={2001},
  volume={21 1},
  pages={115-20}
}
The entire coding region of open reading frame 2 (ORF2) of porcine circovirus 2 (PCV2) was linked to the 3'-end of the maltose-binding protein (MBP)-His(8)-tag gene. The fusion protein was expressed as soluble form after induction by isopropylthio-beta-d-galactoside. MBP-His(8)-ORF2 was purified to homogeneity by immobilized metal affinity chromatography based on the interaction of the polyhistidine-tag with metal ions. Expression could represent 1% of the total protein in Escherichia coli… CONTINUE READING