Bacterial expression and purification of an active ω-atracotoxin-Ar1b from spider Atrax robustus.

@inproceedings{Wang2010BacterialEA,
  title={Bacterial expression and purification of an active ω-atracotoxin-Ar1b from spider Atrax robustus.},
  author={Yu-qin Wang and Akihiro Daikonya and Jian Li and Qiang Liu and Shi-di Huang and Dun Wang},
  year={2010}
}
The ω-atracotoxin-Ar1b toxin (ω-ACTX-Ar1b) is one of the arthropod-selective peptide neurotoxins from the venom of Australian funnel-web spider Atrax robustus. The gene of Ar1b was synthesized and cloned into pET-32a(+) vector to allow expression of Ar1b as a fusion protein with thioredoxin and the His-tag (rTrx-Ar1b) in E. coli BL21 (DE3). The optimal condition for inducing the expression of rTrx-Ar1b was 1.0 mmol L-1 IPTG for 6 h at 28°C. The fusion protein rTrx-Ar1b was expressed in soluble… CONTINUE READING

Topics from this paper.

Similar Papers

Loading similar papers…