Bacterial expression and characterization of human pancreatic phospholipase A2.

@article{Han1997BacterialEA,
  title={Bacterial expression and characterization of human pancreatic phospholipase A2.},
  author={S K Han and Byung In Lee and Wonhwa Cho},
  journal={Biochimica et biophysica acta},
  year={1997},
  volume={1346 2},
  pages={185-92}
}
Mammalian pancreatic phospholipases A2 (PLA2) have recently been implicated in cell surface receptor-mediated inflammation. As a first step toward understanding how human pancreatic PLA2 (hp-PLA2) interacts with membranes and other biological targets including cell-surface receptors, we constructed its bacterial expression vector which can be used for the mutagenesis and protein over-expression. The expression vector (pSH-hp) was constructed using a synthetic hp-PLA2 gene whose transcription is… CONTINUE READING