Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis.

@article{CorralesGarca2013BacterialEA,
  title={Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis.},
  author={Ligia L Corrales-Garc{\'i}a and Ernesto Ortiz and J E Casta{\~n}eda-Delgado and Bruno Rivas-Santiago and Gerardo Corzo},
  journal={Protein expression and purification},
  year={2013},
  volume={89 1},
  pages={33-43}
}
Five variants of human β-defensins (HBDs) were expressed in Escherichia coli using two vector systems (pET28a(+) and pQE30) with inducible expression by IPTG. The last vector has not been previously reported as an expression system for HBDs. The recombinant peptides were different in their lengths and overall charge. The HBDs were expressed as soluble or insoluble proteins depending on the expression system used, and the final protein yields ranged from 0.5 to 1.6 mg of peptide/g of wet weight… CONTINUE READING

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