Bacterial bioluminescence: Isolation and genetic analysis of functions from Vibrio fischeri

  title={Bacterial bioluminescence: Isolation and genetic analysis of functions from Vibrio fischeri},
  author={JoAnne Engebrecht and Kenneth H. Nealson and Michael R. Silverman},
Identification of a locus controlling expression of luminescence genes in Vibrio harveyi
Mutagenesis with transposon mini-Mulac was used to identify loci containing genes for bioluminescence (lux) in the marine bacterium Vibrio harveyi, and the organization and regulation of the lux genes of V.Harveyi are discussed.
Measuring gene expression with light.
Genes in Escherichia coli and Vibrio parahaemolyticus were mutagenized, and mutants containing transposon-generated lux gene fusions produced light as a function of target gene transcription.
Cloning and nucleotide sequence of luxR, a regulatory gene controlling bioluminescence in Vibrio harveyi
Transposon mutagenesis and analysis of the DNA sequence of the cloned DNA indicated that regulatory function resided in a single gene of about 0.6-kilobases named luxR, suggesting a requirement for an additional regulatory component of bioluminescence in recombinant E. coli.
Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis
Ten luxR mutant plasmids were obtained, ten of which encoded full-length but inactive luxR gene products as demonstrated by a Western immunoblot analysis, and the effects of 1 of the 10 mutations could be overcome by the addition of autoinducer at a high concentration.
Transcriptional regulation of lux genes transferred into Vibrio harveyi
The gene transfer system used to transfer lux genes cloned into E. coli back into V. harveyi is useful for expression of proteins as well as for studying the regulation of lux genes in their native environment.
Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi
The constitutive phenotype of LuxO∼ mutants indicates that LuxO acts negatively to control expression of luminescence, and relief of repression by LuxO in the wild type could result from interactions with other components in the Lux signalling system.
Functional identification of the fatty acid reductase components encoded in the luminescence operon of Vibrio fischeri
Results provide direct evidence that the genes coding for the fatty acid reductase polypeptides are an integral part of the luminescent operon in the V. fischeri luminescence system.
Control of the lux regulon of Vibrio fischeri.
Regulation of expression of bioluminescence from the Vibrio fischeri lux regulon in Escherichia coli is a consequence of a unique form of positive feedback superimposed on a poorly defined cis-acting
Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA
The altered ability to express light was found to be due to a mutation in the host and not to an alteration in the recombinant DNA, and appeared to arise in part from production of higher levels of polycistronic mRNAs coding for luciferase.


Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi.
Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
  • M. Casadaban, S. Cohen
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
The lactose structural genes, without the lactose promoter, have been incorporated into the bacteriophage Mu genome to form a Mu-lac specialized transducing phage. This phage also carries a gene
Sensory transducers of E. coli are encoded by homologous genes
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
P15A-derived plasmids were not self-transmissible and were mobilized poorly by Hfr strains; however, mobilization was complemented by the presence of a ColE1 plasmid within the same cell.
Biochemistry of Bacterial Bioluminescence
Cellular Control of the Synthesis and Activity of the Bacterial Luminescent System
In bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth, attributed to a stimulation of existing patterns of synthesis.