Bacterial and plant ketol-acid reductoisomerases have different mechanisms of induced fit during the catalytic cycle.

@article{Wong2012BacterialAP,
  title={Bacterial and plant ketol-acid reductoisomerases have different mechanisms of induced fit during the catalytic cycle.},
  author={Sze-Ho Wong and Thierry G. A. Lonhienne and Donald J Winzor and Gerhard Schenk and Luke W. Guddat},
  journal={Journal of molecular biology},
  year={2012},
  volume={424 3-4},
  pages={168-79}
}
Ketol-acid reductoisomerase (KARI) is the second enzyme in the branched-chain amino acid biosynthesis pathway, which is found in plants, fungi and bacteria but not in animals. This difference in metabolism between animals and microorganisms makes KARI an attractive target for the development of antimicrobial agents. Herein we report the crystal structure of Escherichia coli KARI in complex with Mg(2+) and NADPH at 2.3Å resolution. Ultracentrifugation studies confirm that the enzyme exists as a… CONTINUE READING

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