Bacterial Nanobioreactors--Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles.

@article{Alves2015BacterialNE,
  title={Bacterial Nanobioreactors--Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles.},
  author={Nathan J. Alves and Kendrick B. Turner and Michael A. Daniele and Eunkeu Oh and Igor L. Medintz and Scott A. Walper},
  journal={ACS applied materials & interfaces},
  year={2015},
  volume={7 44},
  pages={24963-72}
}
All bacteria shed outer membrane vesicles (OMVs) loaded with a diverse array of small molecules, proteins, and genetic cargo. In this study we sought to hijack the bacterial cell export pathway to simultaneously produce, package, and release an active enzyme, phosphotriesterase (PTE). To accomplish this goal the SpyCatcher/SpyTag (SC/ST) bioconjugation system was utilized to produce a PTE-SpyCatcher (PTE-SC) fusion protein and a SpyTagged transmembrane porin protein (OmpA-ST), known to be… CONTINUE READING