Bacterial 5-Nucleotidase in Aquatic Ecosystems: A Novel Mechanism of Phosphorus Regeneration

@article{Ammerman1985Bacterial5I,
  title={Bacterial 5-Nucleotidase in Aquatic Ecosystems: A Novel Mechanism of Phosphorus Regeneration},
  author={Jw Ammerman and F. Azam},
  journal={Science},
  year={1985},
  volume={227},
  pages={1338 - 1340}
}
Zooplankton excretion and algal alkaline phosphatase are presumed to be responsible for phosphorus recycling in aquatic ecosystems; the role of bacteria has been unclear. High levels of bacterial cell-surface 5-nucleotidase were discovered in samples of picoplankton from California coastal waters. 5-Nucleotidase rapidly generated orthophosphate from 5-nucleotide added in nanomolar amounts and could supply half the orthophosphate required by plankton. Unlike alkaline phosphatase, 5-nucleotidase… Expand
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References

SHOWING 1-10 OF 20 REFERENCES
Dissolved ATP in the sea and its utilisation by marine bacteria
TLDR
DATP occurs in seawater in significant concentrations and is utilised rapidly by marine bacteria and would be a useful tracer for following the production and fate of labile DOM in seaw water as it is a universal component of the cellular metabolic pools of all living organisms. Expand
Dissolved adenosine triphosphate utilization by free-living and attached bacterioplankton
TLDR
High concentrations of dissolved adenosine triphosphate and rates of its uptake by marine bacteria in seawater from the continental shelf and Gulf Stream off the southeastern USA varied markedly with small-scale temporal and spatial changes. Expand
The Ecological Role of Water-Column Microbes in the Sea*
TLDR
Evidence is presented to suggest that numbers of free bacteria are controlled by nanoplankton~c heterotrophic flagellates which are ubiquitous in the marine water column, thus providing the means for returning some energy from the 'microbial loop' to the conventional planktonic food chain. Expand
The utilization of inorganic and organic phosphorous compounds as nutrients by eukaryotic microalgae: a multidisciplinary perspective: part 1.
TLDR
This comprehensive literature review of the phosphorus nutrition and metabolism of eukaryotic microalgae deals sequentially with extracellular P-compounds available for algal utilization and growth and includes specific macroalgae in considering certain subtopics where such algae were better investigated and provided a good basis for comparison. Expand
Microbial uptake of dissolved organic matter in Mcmurdo Sound, Antarctica
TLDR
In McMurdo Sound, the western side is highly oligotrophic, but the eastern side has an abundant active bacterioplankton, comparable to that of temperate coastal waters. Expand
Multiphasic uptake of D-glucose by an oligotrophic marine bacterium
TLDR
Multiphasic uptake mechanisms in LNB-155 indicate adaptation to living in environments with fluctuating levels of D-glucose, consistent with the hypothesis that pelagic marine bacteria experience considerable fluctuations in nutrient concentration in their microenvironment. Expand
Community metabolism of adenylates by microheterotrophs from the Los Angeles Harbor and Southern California coastal waters
TLDR
Assuming steady-state conditions for the dissolved adenylate pool, it is proposed a model in which losses from this pool are balanced by inputs to the pool through inefficient feeding, lysis and decomposition of particulateAdenylates. Expand
Photosynthesis of picoplankton in the oligotrophic ocean
Against a background of controversy concerning the absolute magnitude of biological productivity in the oligotrophic, tropical ocean1–4, evidence is accumulating for the existence there of aExpand
Alkaline phosphatase activity in subtropical Central North Pacific waters using a sensitive fluorometric method
TLDR
A fluorometric method for assaying low levels of the enzyme alkaline phosphatase in seawater based on the hydrolysis of the monophosphate ester bond of 3-0-methylfluorescein phosphate is described. Expand
Role of membrane-bound 5'-nucleotidase in nucleotide uptake by the moderate halophile Vibrio costicola.
TLDR
Intact cells of Vibrio costicola hydrolyzed ATP, ADP, and AMP and the membrane-bound 5'-nucleotidase was solely responsible for these activities, as shown by experiments with anti-5'nucleotinase serum and with the ATP analog, adenosine 5'-(beta gamma-imido)-diphosphate. Expand
...
1
2
...