Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner

@article{Galburt2007BacktrackingDT,
  title={Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner},
  author={E. Galburt and S. Grill and Anna Wiedmann and L. Lubkowska and Jason Choy and E. Nogales and M. Kashlev and C. Bustamante},
  journal={Nature},
  year={2007},
  volume={446},
  pages={820-823}
}
RNA polymerase II (RNAP II) is responsible for transcribing all messenger RNAs in eukaryotic cells during a highly regulated process that is conserved from yeast to human, and that serves as a central control point for cellular function. Here we investigate the transcription dynamics of single RNAP II molecules from Saccharomyces cerevisiae against force and in the presence and absence of TFIIS, a transcription elongation factor known to increase transcription through nucleosomal barriers… Expand
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