Backbone tracking by the SF2 helicase NPH-II

  title={Backbone tracking by the SF2 helicase NPH-II},
  author={Jane Kawaoka and Eckhard Jankowsky and Anna M Pyle},
  journal={Nature Structural &Molecular Biology},
Members of the DExH/D family of proteins, a subset of helicase superfamily 2 (SF2), are involved in virtually all aspects of RNA metabolism. NPH-II, a prototypical member of this protein family, exhibits robust RNA helicase activity in vitro. Using a series of modified substrates to explore the unwinding mechanism of NPH-II, we observed that the helicase tracks exclusively on the loading strand, where it requires covalent continuity and specifically recognizes the ribose-phosphate backbone. NPH… CONTINUE READING
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