Backbone dynamics of (1-71)- and (1-36)bacterioopsin studied by two-dimensional (1)H- (15)N NMR spectroscopy.

@article{Orekhov1995BackboneDO,
  title={Backbone dynamics of (1-71)- and (1-36)bacterioopsin studied by two-dimensional (1)H- (15)N NMR spectroscopy.},
  author={Vladislav Yu. Orekhov and Konstantin Pervushin and Dmitry M. Korzhnev and Alexander S. Arseniev},
  journal={Journal of biomolecular NMR},
  year={1995},
  volume={6 2},
  pages={113-22}
}
The backbone dynamics of uniformly (15)N-labelled fragments (residues 1-71 and 1-36) of bacterioopsin, solubilized in two media (methanol-chloroform (1:1), 0.1 M (2)HCO(2)NH(4), or SDS micelles) have been investigated using 2D proton-detected heteronuclear (1)H-(15)N NMR spectroscopy at two spectrometer frequencies, 600 and 400 MHz. Contributions of the conformational exchange to the transverse relaxation rates of individual nitrogens were elucidated using a set of different rates of the CPMG… CONTINUE READING