Bacillus subtilis sequence-independent DNA-binding and DNA-bending protein Hbsu negatively controls its own synthesis.

@article{Fernndez1999BacillusSS,
  title={Bacillus subtilis sequence-independent DNA-binding and DNA-bending protein Hbsu negatively controls its own synthesis.},
  author={Silvia Fern{\'a}ndez and Juan Alonso},
  journal={Gene},
  year={1999},
  volume={231 1-2},
  pages={187-93}
}
Transcription of the hbs gene under vegetative growth condition is subject to repression when cells enter in late exponential phase. We have determined the sites at which transcription of the hbs gene initiates in vitro. On a supercoiled template, transcription of the hbs gene is initiated by sigmaARNAP at two overlapping hbs promoters (P1 and P3). We have demonstrated that highly purified Hbsu protein acts as a repressor of its own synthesis. The binding of the sequence-independent DNA-binding… CONTINUE READING