Bacillus sphaericus Penicillin V Acylase: Purification, Substrate Specificity, and Active-Site Characterization

Abstract

Penicillin V acylase from Bacillus sphaericus was purified to homogeneity with an overall yield of 15%. The enzyme exhibited comparatively high specificity for penicillin V, penicillin G, and other related compounds being hydrolyzed at less than 10% of the rate of penicillin V. Moreover, the high rate of hydrolysis was observed when the side chain of the… (More)
DOI: 10.1007/s002849900159

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