BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Abstract

MOTIVATION Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.

DOI: 10.1093/bioinformatics/btv351
0200400201520162017
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@article{Simo2015BUSCOAG, title={BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs}, author={Felipe A. Sim{\~a}o and Robert M. Waterhouse and Panagiotis Ioannidis and Evgenia V. Kriventseva and Evgeny M. Zdobnov}, journal={Bioinformatics}, year={2015}, volume={31 19}, pages={3210-2} }