BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs


MOTIVATION Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.

DOI: 10.1093/bioinformatics/btv351
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@article{Simo2015BUSCOAG, title={BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs}, author={Felipe A. Sim{\~a}o and Robert M. Waterhouse and Panagiotis Ioannidis and Evgenia V. Kriventseva and Evgeny M. Zdobnov}, journal={Bioinformatics}, year={2015}, volume={31 19}, pages={3210-2} }