BRCA1 and BRCA2 mutation screening using SmartCycler II high-resolution melt curve analysis.

@article{Dufresne2006BRCA1AB,
  title={BRCA1 and BRCA2 mutation screening using SmartCycler II high-resolution melt curve analysis.},
  author={Scott D. Dufresne and Dorothy R. Belloni and Wendy A. Wells and Gregory J. Tsongalis},
  journal={Archives of pathology & laboratory medicine},
  year={2006},
  volume={130 2},
  pages={185-7}
}
CONTEXT Real-time polymerase chain reaction technologies have replaced many of the more labor-intense methods in the molecular diagnostics laboratory. Similarly, melt curve analysis can provide a rapid means of mutation screening. OBJECTIVE To determine if real-time polymerase chain reaction and melt curve analysis using the SmartCycler II could be used as a screening tool for 3 common mutations in BRCA1 and BRCA2. DESIGN Real-time polymerase chain reaction amplification with SYBR Green I… CONTINUE READING