BPV1 E2 protein enhances packaging of full-length plasmid DNA in BPV1 pseudovirions.

@article{Zhao2000BPV1EP,
  title={BPV1 E2 protein enhances packaging of full-length plasmid DNA in BPV1 pseudovirions.},
  author={Kong-nan Zhao and Kylie Hengst and Wen Jun Liu and Yue hua Liu and Xiaosong Song Liu and Nigel A. J. McMillan and Ian H Frazer},
  journal={Virology},
  year={2000},
  volume={272 2},
  pages={382-93}
}
We studied determinants of efficient encapsidation of circular DNA, incorporating a PV early region DNA sequence (nt 584-1978) previously shown to enhance packaging of DNA within papillomavirus (PV)-like particles (VLPs). Insect coelomic cells (Sf-9) and cultured monkey kidney cells (Cos-1) were transfected with an 8-kb reporter plasmid incorporating the putative BPV packaging sequence and infected with BPV1 L1 and L2 recombinant baculovirus or vaccinia virus. Heavy (1.34 g/ml) and light (1.30… CONTINUE READING