BP1, a Homeodomain-Containing Isoform of DLX4, Represses the β-Globin Gene

@article{Chase2002BP1AH,
  title={BP1, a Homeodomain-Containing Isoform of DLX4, Represses the $\beta$-Globin Gene},
  author={Michael B. Chase and S Fu and Susanne B. Haga and Gregory J Davenport and Holly Stevenson and Khanh V. Do and Doris A. Morgan and Alex L. Mah and Patricia E. Berg},
  journal={Molecular and Cellular Biology},
  year={2002},
  volume={22},
  pages={2505 - 2514}
}
ABSTRACT In earlier studies we identified a putative repressor of the human β-globin gene, termed beta protein 1 (BP1), which binds to two silencer DNA sequences upstream of the adult human β-globin gene and to a negative control region upstream of the adult δ-globin gene. Further studies demonstrated an inverse correlation between the binding affinity of the BP1 protein for the distal β-globin silencer sequence and the severity of sickle cell anemia, suggesting a possible role for BP1 in… Expand
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TLDR
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TLDR
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A common protein binds to two silencers 5′ to the human β-globin gene
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The results and others presented here suggest that BP1 may act as a repressor protein, which seems to be an important component of tissue and developmental specific globin gene regulation. Expand
Distinct functions of two isoforms of a homeobox gene, BP1 and DLX7, in the regulation of the beta-globin gene.
TLDR
Sequence comparison revealed that BP1 is a member of the distal-less (DLX) family of homeobox genes and that it shares its homeodomain and 3' sequences with another DLX cDNA, DLX7, which strongly suggest thatBP1 andDLX7 are isoforms (derived from the same gene). Expand
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A combination of general DNA-bending and specific transcriptional factors appear to be involved in beta-globin silencing in the embryonic/fetal erythroid stage. Expand
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Binding of HMG‐I(Y) elicits structural changes in a silencer of the human β‐globin gene
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A model in which HMG‐I(Y) alters DNA conformation to allow binding of repressor proteins, and in which the relative amount of H MG‐I (Y) helps to determine the repressive state of the β‐globin gene is presented. Expand
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The results suggest the possibility that the decreased expression of the β‐globin gene exhibited by the carrier may be due, at least in part, to tighter binding of a protein which functions as a negative control element or represser. Expand
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Sequences in the upstream promoter region of the adult beta globin gene contribute to its factor-mediated suppression early in development and then may modulate its expression at a later stage, as well as indicating the presence of a positive transcriptional element in the downstream delta globin regulatory region. Expand
The β-Globin LCR Is Not Necessary for an Open Chromatin Structure or Developmentally Regulated Transcription of the Native Mouse β-Globin Locus
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In the native murine beta-globin locus, the LCR is necessary for normal levels of transcription, but other elements are sufficient to establish the open chromatin structure, transcription, and developmental specificity of the locus. Expand
The gammaPE complex contains both SATB1 and HOXB2 and has positive and negative roles in human gamma-globin gene regulation.
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Antisera directed against either SATB1 or HOXB2 reacted specifically with the entire gammaPE complex in electrophoretic mobility shift assays (EMSAs), suggesting that the two proteins can bind to the gammaPE binding site simultaneously. Expand
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