BOSTON UNIVERSITY SCHOOL OF MEDICINE Thesis EVALUATION OF PHADEBAS FORENSIC PRESS TEST PAPER AS A SOURCE OF BIOLOGICAL MATERIAL FOR IMMUNOCHROMATOGRAPHIC TESTING AND DNA ANALYSIS by NICOLE

Abstract

Saliva is commonly found at crime scenes and other biological fluids, such as semen, urine, blood, and fecal matter may be present alongside saliva on an article of clothing, such as underwear. Forensic testing is required to detect saliva in order to corroborate events related to a crime and to identify stains that can be used in DNA analysis. A cross-reactivity study was carried out to determine the specificity of the Phadebas® Forensic Press test for saliva stains. Whole blood, semen, urine, fecal matter, vaginal secretions, condoms, lubricants, vinegar, and PBS were all tested for reactivity with the Phadebas® paper. Only fecal matter and urine demonstrated cross-reactivity within the 60-minute test window. As conservation of sample is a necessity in forensics, a study was carried out to determine if a cutting from Phadebas® paper performs similarly to a cutting from the original sample or stain in immunochromatographic testing with RSIDTM-Saliva. Testing of extracts from fabric cuttings allowed for detection of 1:100 dilutions of saliva, whereas only neat, 1:5 and 1:10 dilutions were able to be detected when using Phadebas® paper

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Cite this paper

@inproceedings{DAVIDEK2013BOSTONUS, title={BOSTON UNIVERSITY SCHOOL OF MEDICINE Thesis EVALUATION OF PHADEBAS FORENSIC PRESS TEST PAPER AS A SOURCE OF BIOLOGICAL MATERIAL FOR IMMUNOCHROMATOGRAPHIC TESTING AND DNA ANALYSIS by NICOLE}, author={MARIE DAVIDEK and NICOLE DAVIDEK and Amy N. Brodeur and NICOLE MARIE DAVIDEK}, year={2013} }