BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks

@inproceedings{Yan2017BLISSIA,
  title={BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks},
  author={Winston X. Yan and Reza Mirzazadeh and Silvano Garnerone and David J. Scott and Martin W Schneider and Tomasz Kallas and Joaquin Custodio and Erik Wernersson and Yinqing Li and Linyi Gao and Yana Federova and Bernd Zetsche and Feng Zhang and Magda Bienko and Nicola Crosetto},
  booktitle={Nature communications},
  year={2017}
}
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS), featuring the following: (1) direct labelling of DSBs in fixed cells or tissue sections on a solid surface; (2) low-input requirement by linear amplification of tagged DSBs by in vitro transcription; (3… CONTINUE READING
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Breaks labeling in situ and sequencing (BLISS) Protocol Exchange

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Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

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