BLAST and FASTA similarity searching for multiple sequence alignment.

@article{Pearson2014BLASTAF,
  title={BLAST and FASTA similarity searching for multiple sequence alignment.},
  author={William R. Pearson},
  journal={Methods in molecular biology},
  year={2014},
  volume={1079},
  pages={75-101}
}
BLAST, FASTA, and other similarity searching programs seek to identify homologous proteins and DNA sequences based on excess sequence similarity. If two sequences share much more similarity than expected by chance, the simplest explanation for the excess similarity is common ancestry-homology. The most effective similarity searches compare protein sequences, rather than DNA sequences, for sequences that encode proteins, and use expectation values, rather than percent identity, to infer homology… CONTINUE READING