BIPES, a cost-effective high-throughput method for assessing microbial diversity

@article{Zhou2011BIPESAC,
  title={BIPES, a cost-effective high-throughput method for assessing microbial diversity},
  author={Hong-Wei Zhou and Dong-Fang Li and Nora Fung-yee Tam and X Jiang and Hai Zhang and Hua-fang Sheng and Jin Qin and Xiao Nan Liu and Fei Zou},
  journal={The ISME Journal},
  year={2011},
  volume={5},
  pages={741-749}
}
Pyrosequencing of 16S rRNA (16S) variable tags has become the most popular method for assessing microbial diversity, but the method remains costly for the evaluation of large numbers of environmental samples with high sequencing depths. We developed a barcoded Illumina paired-end (PE) sequencing (BIPES) method that sequences each 16S V6 tag from both ends on the Illumina HiSeq 2000, and the PE reads are then overlapped to obtain the V6 tag. The average accuracy of Illumina single-end (SE) reads… CONTINUE READING

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