Corpus ID: 39371379

BIOLUMINESCENT IMAGING OF AN NF-kB TRANSGENIC MOUSE MODEL FOR MONITORING IMMUNE RESPONSE TO A BIOARTIFICIAL PANCREAS REAL TIME AND IN VIVO : VALIDATION OF THE METHOD

@inproceedings{Roth2005BIOLUMINESCENTIO,
  title={BIOLUMINESCENT IMAGING OF AN NF-kB TRANSGENIC MOUSE MODEL FOR MONITORING IMMUNE RESPONSE TO A BIOARTIFICIAL PANCREAS REAL TIME AND IN VIVO : VALIDATION OF THE METHOD},
  author={David J Roth and Taylor G. Wang and Frederick R Haselton and Duco Jansen and Rick Haselton and Michael J Fowler and Timothy S. Blackwell},
  year={2005}
}
Cell encapsulation is a novel therapeutic approach for the treatment of Type I Diabetes Mellitus that circumvents both the immunosuppression and limited allograft donor source dilemma. Current methods for scoring the biocompatibility of the alginatebased capsules that sequester Islets of Langerhans include fabrication and implantation into the peritoneal cavity of mice, incubation for specified periods of time, retrieval via peritoneal lavage, and observation of the number of cells or cell… Expand
Transgenic Animal Models to Visualize Cancer-Related Cellular Processes by Bioluminescence Imaging
TLDR
A review of the available models to visualized cancer and cancer-associated events in living animals by BLI and as they have been successful in identifying new stages of early tumor progression, new interactions between different tissues and new therapeutic responsiveness is provided. Expand
Molecular imaging of pancreatic islet transplantation.
TLDR
Islet replacement therapy, pancreatic islet transplantation, is considered as a potential option for curing T1DM, with ongoing researches focusing on islet availability, appropriate sites for implantation, new methods using biomaterials (e.g. microencapsulation), immune modulation and more. Expand
Improving the Procedure for Detection of Intrahepatic Transplanted Islets by Magnetic Resonance Imaging
  • M. Malosio, A. Esposito, +12 authors A. Del Maschio
  • Medicine
  • American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
  • 2009
TLDR
With this standardized labeling procedure, labeled islets are neither inflamed nor more susceptible to inflammatory insults than unlabeled ones, and this report represents an important contribution towards the development of a standardized and safe clinical protocol for the noninvasive imaging of transplanted islets in humans. Expand
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Investigation of reporter induction for up to 90 days in NF-κB transgenic reporter mice following intravenous injection of PEG-coated AuNPs suggested that inflammation resulted in mild fibrosis and affected hepatic function and further investigation is required to fully assess the long-term impacts by P EG-AuNPs on liver health. Expand
Imaging of Gene Expression in Live Pancreatic Islet Cell Lines Using Dual-Isotope SPECT
TLDR
Dual-isotope SPECT is a promising method to detect gene expression in and location of transplanted pancreatic cells in vivo and confirmed the presence of glucagon- and insulin-positive cells at the site of transplantation. Expand
TLR4 Mediates Early Graft Failure After Intraportal Islet Transplantation
  • Q. Gao, L. Ma, X. Gao, W. Yan, P. Williams, D. Yin
  • Medicine
  • American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
  • 2010
TLDR
Inhibition of TLR4 activation represents a novel strategy to attenuate early graft failure following intraportal islet transplantation, as evidenced by reduced TNFα and IL‐1ß production, and improved islet viability. Expand
High-Resolution Magnetic Resonance Imaging Quantitatively Detects Individual Pancreatic Islets
TLDR
Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo. Expand
Non‐invasive detection of transplanted pancreatic islets
TLDR
This review will focus on the latest advancements in the field of in vivo imaging of islet transplantation and describe various islet labelling and imaging techniques and critically look into limitations and obstacles currently present on the way to successful clinical implementation of this approach. Expand
PET probes for imaging pancreatic islet cells
TLDR
This review summarized the latest developments of PET probes which are targeting proteins including GLP-1, VMAT2, GPR44, antigen, glucokinase and reporter genes in the beta cell for imaging pancreatic islet cells. Expand
Effects of Mn-DPDP and manganese chloride on hemodynamics and glucose tolerance in anesthetized rats
TLDR
Mn-DPDP administration does not affect glucose tolerance or regional blood flow, besides an increase in arterial hepaticBlood flow, and may therefore be suitable for visualization of islets. Expand
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Alginate/poly-L-lysine capsules do not appear to be biocompatible and may account for the variable results in islet graft survival found with these capsules, which could be inhibited by immunosuppressive agents. Expand
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This discussion concentrates on three antigen-specific immunologic processes that impede the application of islet transplantation as a therapy for insulin-dependent diabetes: Allograft immunity, Xenograft Immunity, and Autoimmune pathogenesis of Type I diabetes. Expand
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