BID-D59A is a potent inducer of apoptosis in primary embryonic fibroblasts.

  title={BID-D59A is a potent inducer of apoptosis in primary embryonic fibroblasts.},
  author={Rachel Sarig and Yehudit Zaltsman and Richard Marcellus and Richard Flavell and Tak W Mak and Atan Gross},
  journal={The Journal of biological chemistry},
  volume={278 12},
The proapoptotic activity of BID seems to solely depend upon its cleavage to truncated tBID. Here we demonstrate that expression of a caspase-8 non-cleavable (nc) BID-D59A mutant or expression of wild type (wt) BID induces apoptosis in Bid -/-, caspase-8 -/-, and wt primary MEFs. Western blot analysis indicated that no cleavage products appeared in cells expressing ncBID. ncBID was as effective as wtBID in inducing cytochrome c release, caspase activation, and apoptosis. ncBID and wtBID (nc… CONTINUE READING

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