BIA/MS of epitope-tagged peptides directly from E. coli lysate: multiplex detection and protein identification at low-femtomole to subfemtomole levels.

Abstract

The use of biomolecular interaction analysis mass spectrometry to selectively isolate, detect, and characterize epitope-tagged peptides present in total cell lysates is demonstrated. Epitope-tagged tryptic peptides were captured via affinity interactions with either chelated Ni2+ or monoclonal antibodies and detected using surface plasmon resonance… (More)

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@article{Nelson1999BIAMSOE, title={BIA/MS of epitope-tagged peptides directly from E. coli lysate: multiplex detection and protein identification at low-femtomole to subfemtomole levels.}, author={Randall W. Nelson and Jonathan W. Jarvik and Bruce E. Taillon and Kemmons A. Tubbs}, journal={Analytical chemistry}, year={1999}, volume={71 14}, pages={2858-65} }