BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

@article{Canver2015BCL11AED,
  title={BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis},
  author={Matthew C Canver and E. Smith and F. Sher and L. Pinello and Neville E. Sanjana and O. Shalem and Diane D. Chen and Patrick G. Schupp and Divya S Vinjamur and S. P. Garc{\'i}a and S. Luc and Ryo Kurita and Y. Nakamura and Y. Fujiwara and T. Maeda and Guocheng Yuan and Z. Feng and S. Orkin and D. Bauer},
  journal={Nature},
  year={2015},
  volume={527},
  pages={192 - 197}
}
  • Matthew C Canver, E. Smith, +16 authors D. Bauer
  • Published 2015
  • Biology, Medicine
  • Nature
  • Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of human BCL11A, subject to common genetic variation associated with the fetal haemoglobin level, the mouse orthologue of which is necessary for erythroid BCL11A expression. Here we develop pooled… CONTINUE READING
    479 Citations
    A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening.
    • 84
    • PDF
    Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis
    • 24
    Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing
    • 14
    • PDF
    Nucleosomes impede Cas9 access to DNA in vivo and in vitro
    • 229
    Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations
    • 12
    • PDF

    References

    SHOWING 1-10 OF 80 REFERENCES
    In vivo enhancer analysis of human conserved non-coding sequences
    • 1,078
    • PDF
    Massively parallel functional dissection of mammalian enhancers in vivo
    • 419
    • PDF
    ChIP-seq accurately predicts tissue-specific activity of enhancers
    • 1,508
    • PDF
    The accessible chromatin landscape of the human genome
    • 2,160
    • PDF
    Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
    • 978
    • PDF
    Developmental and species-divergent globin switching are driven by BCL11A
    • 319
    • PDF