BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

  title={BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis},
  author={Matthew C Canver and E. Smith and F. Sher and L. Pinello and Neville E. Sanjana and O. Shalem and Diane D. Chen and Patrick G. Schupp and Divya S Vinjamur and S. P. Garc{\'i}a and S. Luc and Ryo Kurita and Y. Nakamura and Y. Fujiwara and T. Maeda and Guocheng Yuan and Z. Feng and S. Orkin and D. Bauer},
  pages={192 - 197}
  • Matthew C Canver, E. Smith, +16 authors D. Bauer
  • Published 2015
  • Biology, Medicine
  • Nature
  • Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of human BCL11A, subject to common genetic variation associated with the fetal haemoglobin level, the mouse orthologue of which is necessary for erythroid BCL11A expression. Here we develop pooled… CONTINUE READING
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