BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage.

@article{Placantonakis2009BACTI,
  title={BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage.},
  author={Dimitris G Placantonakis and Mark J. Tomishima and Fabien Gilbert Lafaille and Sabrina C Desbordes and Fan Jia and Nicholas D. Socci and Agn{\`e}s Viale and Hyojin Lee and Neil Harrison and V Tabar and Lorenz P Studer},
  journal={Stem cells},
  year={2009},
  volume={27 3},
  pages={
          521-32
        }
}
Human embryonic stem cells (hESCs) have enormous potential for applications in basic biology and regenerative medicine. However, harnessing the potential of hESCs toward generating homogeneous populations of specialized cells remains challenging. Here we describe a novel technology for the genetic identification of defined hESC-derived neural cell types using bacterial artificial chromosome (BAC) transgenesis. We generated hESC lines stably expressing Hes5::GFP, Dll1::GFP, and HB9::GFP BACs… CONTINUE READING
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