B2 RNA binds directly to RNA polymerase II to repress transcript synthesis

@article{Espinoza2004B2RB,
  title={B2 RNA binds directly to RNA polymerase II to repress transcript synthesis},
  author={Celso A. Espinoza and Tiffany A Allen and Aaron Hieb and Jennifer F. Kugel and James A. Goodrich},
  journal={Nature Structural \&Molecular Biology},
  year={2004},
  volume={11},
  pages={822-829}
}
B2 RNA is a small noncoding RNA polymerase III transcript that represses mRNA transcription in response to heat shock in mouse cells. Here we define the mechanism by which B2 RNA inhibits RNA polymerase II (Pol II) transcription. Using a purified Pol II transcription system, we found that B2 RNA potently inhibits transcription by binding to core Pol II with high affinity and specificity. Through this interaction, B2 RNA assembles into preinitiation complexes at the promoter and blocks RNA… 
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During the heat shock response in mouse cells, a small noncodingRNA polymerase III transcript, B2 RNA, associates with RNA polymerase II and represses transcription of specific mRNA genes, defining a unique transcriptional regulatory mechanism involving an RNA regulator and revealing how mRNA transcription is repressed upon heat shock.
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