B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli.

@article{Jordan1997B12dependentRR,
  title={B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli.},
  author={Albert Jordan and Eduard Torrents and Christian Jeanthon and Rolf Eliasson and Ulf Hellman and Christer Wernstedt and Jordi Barb{\'e} and Isidre Gibert and Peter A Reichard},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 25},
  pages={13487-92}
}
The ribonucleotide reductases from three ancient eubacteria, the hyperthermophilic Thermotoga maritima (TM), the radioresistant Deinococcus radiodurans (DR), and the thermophilic photosynthetic Chloroflexus aurantiacus, were found to be coenzyme-B12 (class II) enzymes, similar to the earlier described reductases from the archaebacteria Thermoplasma acidophila and Pyrococcus furiosus. Reduction of CDP by the purified TM and DR enzymes requires adenosylcobalamin and DTT. dATP is a positive… CONTINUE READING

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