B cells express intracellular but not surface receptors for testosterone and estradiol

@article{Benten2002BCE,
  title={B cells express intracellular but not surface receptors for testosterone and estradiol},
  author={W. Peter M. Benten and Christian Stephan and Frank Wunderlich},
  journal={Steroids},
  year={2002},
  volume={67},
  pages={647-654}
}
Increasing evidence indicates the existence of membrane receptors for testosterone (mAR) and estradiol (mER) on the surface of cells, besides the classic intracellular androgen receptor (iAR) and estrogen receptors (iER). Here, we investigate the occurrence of sex steroid receptors in B cells isolated from the spleen of C57BL/10 mice using magnetic cell sorting. RT-PCR reveals the presence of iAR, iERalpha, but not iERbeta. Using different anti-iAR and anti-iER antibodies flow cytometry and… Expand
Estrogen augments the T cell-dependent but not the T-independent immune response
TLDR
The results provide the first evidence that estrogen enhances the TD but not the TI-2 response, and show that estrogen may have a direct impact on B and T cells by inducing rapid signaling events, such as Erk and AKT phosphorylation, cell-specific Ca2+ signal, and NFκB activation. Expand
Immunomodulation by estrogen and estren
Estrogen affects the development and regulation of the immune system. Treatment of gonadectomized mice with estrogen results in suppression of T and B lymphopoiesis, as well as decreased delayed typeExpand
Migration of mouse antibody-secreting hybridoma cells from blood to genital tract and its regulation by sex hormones are associated with the differential expression patterns of adhesion molecules and chemokines in the tract rather than in the antibody-secreting cells.
TLDR
Pre preferential migration of ASCs into female genital tract and regulation of migration by sex hormones are associated with expression patterns of adhesion molecules and chemokines in genital tract rather than in ASCs. Expand
A dynamic network of estrogen receptors in murine lymphocytes: fine‐tuning the immune response
TLDR
It is shown that in lymphocytes, mERs can signalize themselves, and upon ligation, they are able to coordinate translocation of other E2Rs to the PM, implying existence of a complex, dynamic network of at least seven ER forms in murine lymphocytes: cytoplasmic and membrane‐linked forms of ERα, ERβ, or GPR30 and a mER that can receive extracellular E2 signals. Expand
Oestrogen receptor specificity in oestradiol‐mediated effects on B lymphopoiesis and immunoglobulin production in male mice
TLDR
Results clearly show that both ERα and ERβ are required for complete down‐regulation of B lymphopoiesis while only ERα is needed to up‐regulate immunoglobulin production in both bone marrow and spleen. Expand
Localization of Estrogen Receptor in the Central Lymphoid Organs of Chickens during the Late Stage of Embryogenesis
TLDR
It is indicated that estrogen affects the differentiation of thymic and bursal lymphocytes, suggesting that the underlying role for estrogen in immune function is not fully understood. Expand
Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood mononuclear cells and up‐regulated after in vitro stimulation and in patients with rheumatoid arthritis
  • B. Bartholome, C. Spies, +9 authors F. Buttgereit
  • Biology, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2004
TLDR
For the first time it is demonstrated that mGCR are indeed physiologically present in healthy blood donors, but remained unidentified by conventional techniques due to their small number per cell and actively up‐regulated and transported through the cell after immunostimulation. Expand
Morphological relationships and leukocyte influence on steroid production in the epigonal organ‐ovary complex of the skate, Leucoraja erinacea
TLDR
In this study, the histological and vascular relationships of the EO and ovarian follicles of the little skate, Leucoraja erinacea, were assessed and provide a basis for further investigations of leukocyte secreted factors and cell contact modulation of follicular steroid production. Expand
Expression of the estrogen receptor in blood neutrophils of dairy cows during the periparturient period.
TLDR
The investigation of the presence of estrogen receptors in bovine neutrophils found a clear positive signal and the influence of endogenous 17beta-estradiol and progesterone levels was assessed. Expand
Influence of oestrogen receptor α and β on the immune system in aged female mice
Oestrogen has a dichotomous effect on the immune system. T and B lymphopoiesis in thymus and bone marrow is suppressed, whereas antibody production is stimulated by oestrogen. In this study theExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 65 REFERENCES
Functional testosterone receptors in plasma membranes of T cells
TLDR
The testosterone responsiveness of murine splenic T cells in vitro as well as the presence of unconventional cell surface receptors for testosterone and classical intracellular AR are demonstrated, suggesting that the classical AR present in splenicT cells are not active in the genomic pathway. Expand
Testosterone signaling through internalizable surface receptors in androgen receptor-free macrophages.
TLDR
The data provide evidence for the existence of G-protein-coupled, agonist-sequestrable receptors for testosterone in plasma membranes, which initiate a transcription-independent signaling pathway of testosterone. Expand
Estradiol Signaling via Sequestrable Surface Receptors.
TLDR
The E2-signaling initiated at the plasma membrane is mediated through neither ERα nor ERβ, but rather through a novel G protein-coupled membrane E2 -receptor as revealed by RT-PCR, flow cytometry, and confocal laser scanning microscopy. Expand
Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-α
TLDR
The characteristics of mERalpha and the membrane receptor for glucocorticoids have many similarities, suggesting that this mode of subcellular location/function alternative might be used by other members of the gene family. Expand
Membrane estrogen receptors identified by multiple antibody labeling and impeded‐ligand binding
  • T. Pappas, B. Gametchu, C. Watson
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1995
TLDR
It is demonstrated that GH3/B6 rat pituitary tumor cells contain a membrane ER (mER), and confocal scanning laser microscopy of cells labeled live with the anti‐peptide antibody further supports a membrane localization of ER, suggesting that mER may be structurally similar to iER. Expand
Cell membrane and nuclear estrogen receptors (ERs) originate from a single transcript: studies of ERalpha and ERbeta expressed in Chinese hamster ovary cells.
The existence of a putative membrane estrogen receptor (ER) has been supported by studies accomplished over the past 20 yr. However, the origin and functions of this receptor are not well defined. ToExpand
Identification and characterization of a novel functional estrogen receptor on human sperm membrane that interferes with progesterone effects.
TLDR
The results demonstrate that 17betaE2 induces a rapid and sustained increase of intracellular calcium concentrations ([Ca2+]i), and this effect is totally dependent on the presence of extracellular calcium, because it is completely abolished in a calcium-depleted medium. Expand
Estrogen and progesterone receptors in some human myeloma cell lines and murine hybridomas.
TLDR
The expression of receptors for androgens, estradiol and progesterone by human cell lines of the B lymphocyte lineage and by murine myeloma or hybridomas is investigated and the possible functional role of these sex hormone binding sites in cell proliferation and immunoglobulin secretion deserves further investigation. Expand
Sex steroid receptors in peripheral T cells: absence of androgen receptors and restriction of estrogen receptors to OKT8-positive cells.
TLDR
The restriction of estrogen receptors to T cells bearing the "suppressor-cytotoxic" phenotype suggests a possible pathway for the modulation of T cell suppressive activities by estrogens. Expand
Estradiol binding to cell surface raises cytosolic free calcium in T cells
TLDR
The data suggest a novel E2‐signalling pathway in T cells which is not mediated through the classical nuclear estrogen receptor response but rather through putative plasma membrane receptors for E2, which is inducible by plasma membrane impermeable E2 conjugated to BSA. Expand
...
1
2
3
4
5
...