B-cell epitopes of the allergen Chi t 1.01: peptide mapping of epitopes recognized by rabbit, murine, and human antibodies.

@article{Kampen2001BcellEO,
  title={B-cell epitopes of the allergen Chi t 1.01: peptide mapping of epitopes recognized by rabbit, murine, and human antibodies.},
  author={Vera van Kampen and Verena Liebers and I Sander and Zhiping Chen and Xaver Baur and Monika Raulf-Heimsoth and Frank W. Falkenberg},
  journal={Allergy},
  year={2001},
  volume={56 2},
  pages={118-25}
}
BACKGROUND Chi t 1.01, a hemoglobin of the midge Chironomus thummi thummi, is a widespread environmental and occupational allergen. The aim of the present investigation was to identify and compare peptides involved in B-cell epitopes of Chi t 1.01 recognized by 15 human IgE sera, six murine monoclonal antibodies (mAbs), and a polyclonal rabbit antiserum. METHODS Synthetic peptides 19-21 amino acids long covering the whole Chi t 1.01-sequence were covalently coupled to activated paper disks as… CONTINUE READING

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