Corpus ID: 161601354

Búsqueda, clonación y expresión de genes de carotenogénesis

  title={B{\'u}squeda, clonaci{\'o}n y expresi{\'o}n de genes de carotenog{\'e}nesis},
  author={Araya Garay and Jos{\'e} Miguel.},
Un criterio fundamental de aceptacion de algunos peces de interes comercial como salmonidos es el impacto visual dado por la coloracion rojo-rosado, rojo-anaranjado de la carne, esta coloracion contribuye significativamente a la imagen y puede tener un gran valor, como indicador de calidad del producto. Los salmonidos no pueden sintetizar carotenoides de novo, el color de la carne es obtenido por la absorcion y deposito de carotenoides provenientes de la dieta. Los carotenoides pueden ser… Expand


Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts.
It has been observed in the yeasts S. cerevisiae and C. utilis carrying the lycopene biosynthesis genes that ergosterol content is decreased by 10 and 35%, respectively, and it is likely that the carbon flux for the ergosterols biosynthesis has been partially directed from farnesyl pyrophosphate to a new pathway for the lyCopenoid biosynthesis. Expand
Production of the Carotenoids Lycopene, β-Carotene, and Astaxanthin in the Food Yeast Candida utilis
ABSTRACT The food-grade yeast Candida utilis has been engineered to confer a novel biosynthetic pathway for the production of carotenoids such as lycopene, β-carotene, and astaxanthin. The exogenousExpand
Biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (Citrus sinensis L.) during fruit development and maturation.
Results indicated that PDS gene expression correlated with carotenoid content in developing fruit and that up-regulation of PSY and ZDS genes at the onset of fruit coloration would enhance the production of linear carotenes and the flux into the pathway. Expand
Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein?
A bifunctional β-carotene hydroxylase-ketolase activity has been proposed for the CrtS protein, and the evidence for and against this hypothesis is analyzed in detail in this review. Expand
Virtually complete conversion of lycopene into β-carotene in fruits of tomato plants transformed with the tomato lycopene β-cyclase (tlcy-b) cDNA
Results demonstrate the feasibility of engineering tomato to divert carotenoid metabolic flux toward a desired useful end-product. Expand
Increased Carotenoid Production by the Food YeastCandida utilis through Metabolic Engineering of the Isoprenoid Pathway
Through metabolic engineering of the isoprenoid pathway, a sevenfold increase in the yield of lycopene has been achieved through modifications in related biochemical pathways can be utilized to enhance the production of commercially desirable compounds such as carotenoids. Expand
β-Carotene production by Saccharomyces cerevisiae with regard to plasmid stability and culture media
Time course experiments demonstrated high plasmid stability even over extended cultivation periods, and growth experiments of a specific erg12 deletion mutant showed that the mevalonate kinase (MvaK1) was able to complement the function of the deleted native meValonate Kinase (Erg12) from S. cerevisiae in the MVA pathway under control of the constitutive adh1 promoter. Expand
Metabolic Engineering of Saccharomyces cerevisiae for Astaxanthin Production and Oxidative Stress Tolerance
Interestingly, these ketocarotenoids conferred oxidative stress tolerance on S. cerevisiae cells and has potential for overproduction of astaxanthin and breeding of novel oxidative stress-tolerant yeast strains. Expand
Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin
Two carotenoid ketolase genes were cloned from the cyanobacterium, Nostoc punctiforme PCC 73102 and functionally characterized and upon expression in Escherichia coli, both genes mediated the conversion of β-carotene to canthaxanthin, however in a zeaxanthine-producing E. coli, only the gene product of crtW148 was unable to catalyze this reaction. Expand
Carotenoid Production by Lactoso-Negative Yeasts Co-Cultivated with Lactic Acid Bacteria in Whey Ultrafiltrate
Two strains were selected - the lactoso-negative yeast Rhodotorula rubra GED2 and the homofermentative Lactobacillus casei subsp. casei Ha1 for co-cultivation in cheese whey ultrafiltrate (WU) andExpand