Azido push-pull fluorogens photoactivate to produce bright fluorescent labels.

Abstract

Dark azido push-pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported… (More)
DOI: 10.1021/jp907080r

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