Axonal lesion-induced microglial proliferation and microglial cluster formation in the mouse

@article{DissingOlesen2007AxonalLM,
  title={Axonal lesion-induced microglial proliferation and microglial cluster formation in the mouse},
  author={Lasse Dissing-Olesen and Rune Ladeby and Helle Hvilsted Nielsen and Henrik Toft-hansen and Ishar Dalmau and Bente Finsen},
  journal={Neuroscience},
  year={2007},
  volume={149},
  pages={112-122}
}
Microglia are innate immune cells and form the first line of defense of the CNS. Proliferation is a key event in the activation of microglia in acute pathology, and has been extensively characterized in rats, but not in mice. In this study we investigated axonal-lesion-induced microglial proliferation and surface antigen expression in C57BL/6 mice. Transection of the entorhino-dentate perforant path projection results in an anterograde axonal and a dense terminal degeneration that induces a… Expand
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References

SHOWING 1-10 OF 37 REFERENCES
CXCR3-Dependent Microglial Recruitment Is Essential for Dendrite Loss after Brain Lesion
TLDR
It is shown that CXCR3 signaling is crucial in microglia recruitment but not proliferation, and this recruitment is an essential element for neuronal reorganization. Expand
Lesion of the rat entorhinal cortex leads to a rapid microglial reaction in the dentate gyrus
TLDR
The data suggest that axonal degeneration represents a sufficient stimulus for inducing microglia activation and proliferation in the deafferented dentate gyrus and these activated microglial cells are characterized by immunophenotypes different from those observed in other types of CNS injury. Expand
Microglial cell population dynamics in the injured adult central nervous system
TLDR
The demonstration of an injury-enhanced recruitment of bone marrow-derived cells into the perforant path-denervated dentate gyrus, raises the possibility of using genetically manipulated cells as vectors for lesion-site-specific gene therapy even in minimally injured areas of the central nervous system. Expand
Proliferating resident microglia express the stem cell antigen CD34 in response to acute neural injury
TLDR
The results suggest lesion‐reactive microglia to consist of functionally distinct subpopulations of cells; a major population of activated resident CD34+Mac‐1+microglia with a high capacity for self‐renewal, and a subpopulation of CD34−Mac‐ 1+ micro glia which has a mixed extrinsic and intrinsic origin and whose proliferative capacity is unknown. Expand
Circulating monocytic cells infiltrate layers of anterograde axonal degeneration where they transform into microglia
  • I. Bechmann, J. Goldmann, +6 authors J. Priller
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2005
TLDR
It is demonstrated that blood‐derived monocytic cells infiltrate not only the site of mechanical lesion, but also the layers of anterograde axonal degeneration, where they readily transform into microglia‐like elements. Expand
Induction of microglial immunomolecules by anterogradely degenerating mossy fibres in the rat hippocampal formation
TLDR
It is demonstrated that myelin debris is neither necessary nor sufficient to induce expression of microglial MHC class II antigen within the hippocampus. Expand
Tumor necrosis factor and its p55 and p75 receptors are not required for axonal lesion‐induced microgliosis in mouse fascia dentata
TLDR
Results showed a lower than expected lesion‐induced increase in TNF protein, and that neither TNF nor its receptors were required for the axonal lesion-induced microglial morphological transformation and proliferation or for the initial clearance of degenerated myelin in the PP‐deafferented fascia dentata. Expand
Microglia: a sensor for pathological events in the CNS
TLDR
An understanding of intercellular signalling pathways for microglia proliferation and activation could form a rational basis for targeted intervention on glial reactions to injuries in the CNS. Expand
Microglial reactivity correlates to the density and the myelination of the anterogradely degenerating axons and terminals following perforant path denervation of the mouse fascia dentata
TLDR
The finding of a potentiated or accelerated microglia activation in the medial as compared to the lateral perforant path zone suggests different kinetics of microglial activation in areas with degenerating myelinated and unmyelinated fibers. Expand
Reactive microgliosis engages distinct responses by microglial subpopulations after minor central nervous system injury
TLDR
The results show that distinct subpopulations of microglia respond to minor CNS injury, and the heterogeneity in microglial response may have functional consequences for repair and possibly therapy. Expand
...
1
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3
4
...