Axial tomographic confocal fluorescence microscopy.

  title={Axial tomographic confocal fluorescence microscopy.},
  author={R. Heintzmann and Christoph Cremer},
  journal={Journal of microscopy},
  volume={206 Pt 1},
By physical rotation of the sample, axial tomography enables the acquisition of otherwise inaccessible spatial information from an object. In combination with confocal microscopy, the method can fundamentally improve the effective three-dimensional (3D) resolution. In this report we present a novel method for high resolution reconstruction of confocal axial tomographic data. The method automatically determines the relative angles of rotation, aligns the data from different rotational views and… CONTINUE READING

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