• Biology, Medicine
  • Published in
    The Southeast Asian journal…
    1993

Axenic cultivation of Entamoeba histolytica from liver abscess and its zymodeme.

@article{Thammapalerd1993AxenicCO,
  title={Axenic cultivation of Entamoeba histolytica from liver abscess and its zymodeme.},
  author={Nitaya Thammapalerd and S. Tharavan{\'i}j and Yupaporn Wattanagoon},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={1993},
  volume={24 3},
  pages={
          480-3
        }
}
A local strain of Entamoeba histolytica, the HTH-56: MUTM from a human liver abscess was successfully axenized. The culture was initially established monoxenically in Diamond's TYI-S-33 medium in the presence of Crithidia luciliae and maintained at 34 +/- 0.5 degrees C. After 5 passages it was adapted to axenic cultivation by addition of 0.02% Bacto agar in Diamond's TYI-S-33 medium in place of Crithidia. Subcultures or replacement with fresh complete media were done twice or thrice for 7 days… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 20 REFERENCES

The cultivation of Entamoeba histolytica

WC Boeck, J. Drbohlav
  • Am J Hyg 1925;
  • 1925
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Monoclonal antibodybased immunohistochemical demonstration of En ­ tamoeba histolytica in liver tissue of experiment ­ ally infected hamster ( Mesocricetus auretes )

JB Sherchand, N Thammapalerd, M Riganti, B Punpoowong
  • Int J Parasitol
  • 1993

A serum-free, partially dermed medium, PDM-805, for axenic cultivation of Enta­ moeba histolytica Schaudinn, 1903 and other Enta­ moeba

LS Diamond, Cunnick Cc
  • J Protozoo/1991;
  • 1991

Biomedical applica­ tions of monoclonal antibodies (McAb) against E. histolytica

N Thammapalerd, S. Tharavanij
  • Maramorosch K, ed. Biotechnology for Biological Control of Pests and Vectors. Boca Raton, Florida: CRC Press,
  • 1991

Zymodemes of Entamoeba histolytica.

  • Parasitology today
  • 1987
VIEW 1 EXCERPT

Production of monoclonal antibo­ dies against E. histolytica antigen extract

D Kotimanusvanij, N Thammapalerd, S Khusmith, S. Tharavanij
  • A paper presented at the Annual Current Research Work of The Faculty of Tropical Medicine,
  • 1984