Award Number: W81XWH-12-1-0318 TITLE: Compound 49b Reduces Inflammatory Markers and Apoptosis after Ocular Blast Injury PRINCIPAL INVESTIGATOR:

@inproceedings{MoralesTirado2015AwardNW,
  title={Award Number: W81XWH-12-1-0318 TITLE: Compound 49b Reduces Inflammatory Markers and Apoptosis after Ocular Blast Injury PRINCIPAL INVESTIGATOR:},
  author={Vanessa M. Morales-Tirado},
  year={2015}
}
Loss of functional retinal ganglion cells (RGC) is an element of retinal degeneration that is poorly understood. This is in part due to the lack of a reliable and validated protocol for the isolation of primary RGCs. Here, we optimize a feasible, reproducible, standardized flow cytometry-based protocol for the isolation and enrichment of homogeneous RGC with the Thy1.2 hi CD48 neg CD15 neg CD57 neg surface phenotype. A three-step validation process was performed by (1) genomic profiling of 25… CONTINUE READING

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