Avoiding Proteolysis During Protein Purification.

  title={Avoiding Proteolysis During Protein Purification.},
  author={Barry J. Ryan and Gary T. M. Henehan},
  journal={Methods in molecular biology},
All cells contain proteases which hydrolyze the peptide bonds between amino acids in a protein backbone. Typically, proteases are prevented from nonspecific proteolysis by regulation and by their physical separation into different subcellular compartments; however, this segregation is not retained during cell lysis, which is the initial step in any protein isolation procedure. Prevention of proteolysis during protein purification often takes the form of a two-pronged approach; firstly… CONTINUE READING