Avian influenza virus isolation and propagation in chicken eggs.

@article{Woolcock2008AvianIV,
  title={Avian influenza virus isolation and propagation in chicken eggs.},
  author={Peter R. Woolcock},
  journal={Methods in molecular biology},
  year={2008},
  volume={436},
  pages={35-46}
}
The avian influenza (AI) virus is usually isolated and propagated by inoculating either swab or tissue samples from infected birds into the chorioallantoic sac of embryonating chicken eggs. This is the accepted method, but occasionally an isolation may only be successful when inoculated either into the yolk sac or onto the chorioallantoic membrane of embryonating chicken eggs. Chorioallantoic fluid is harvested from eggs with dead or dying embryos and is tested for the presence of… CONTINUE READING

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