Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation.

@article{Schneider2005AuxotrophicMP,
  title={Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation.},
  author={J. Carrie Schneider and Annika F Jenings and Deborah M Mun and Patricia M Mcgovern and Lawrence Chew},
  journal={Biotechnology progress},
  year={2005},
  volume={21 2},
  pages={343-8}
}
The use of antibiotic-resistance genes as selectable markers in transgenic organisms is coming under increased scrutiny, for fear that they may spread to human pathogens, thereby reducing the effectiveness of antibiotic therapy. A current Pseudomonas fluorescens protein expression system uses a tetracycline resistance gene (tetR/tetA) to maintain an expression plasmid under control of a repressible promoter and a kanamycin resistance gene (kanR) to maintain a plasmid carrying a repressor gene… CONTINUE READING
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