Autoradiography and immunofluorescence combined for autecological study of single cell activity with Nitrobacter as a model system.

@article{Fliermans1975AutoradiographyAI,
  title={Autoradiography and immunofluorescence combined for autecological study of single cell activity with Nitrobacter as a model system.},
  author={Carl B. Fliermans and E. D. L. Schmidt},
  journal={Applied microbiology},
  year={1975},
  volume={30 4},
  pages={676-84}
}
Specific detection of a particular bacterium by immunofluorescence was combined with estimation of its metabolic activity by autoradiography. The nitrifying bacteria Nitrobacter agilis and N. winogradskyi were used as a model system. Nitrobacter were incubated with NaH14CO3 and 14CO2 prior to study. The same preparations made for autoradiograms were stained… CONTINUE READING