Autoradiographic visualization of the natriuretic peptide receptor-B in rat tissues.

@article{Konrad1992AutoradiographicVO,
  title={Autoradiographic visualization of the natriuretic peptide receptor-B in rat tissues.},
  author={Edvard Konrad and Ga{\'e}tan Thibault and Ernesto L Schiffrin},
  journal={Regulatory peptides},
  year={1992},
  volume={39 2-3},
  pages={177-89}
}
Natriuretic peptide receptor-B (NPRB) was visualized in rat tissues by in vitro autoradiography, using its putative physiological agonist C-type natriuretic peptide (CNP). In initial studies, we determined that atrial natriuretic peptide (ANP) is not a suitable ligand for labeling the NPRB: in tissues reported to contain NPRB transcripts, CNP did not inhibit [125I]ANP binding except to NPRC sites. Therefore, to visualize the NPRB we used 125I[Tyr(o)]-CNP as a radioligand with an excess of NPRC… CONTINUE READING

Similar Papers

Loading similar papers…