Autoradiographic localization of serum retinol-binding protein in rat testis.

@article{McGuire1981AutoradiographicLO,
  title={Autoradiographic localization of serum retinol-binding protein in rat testis.},
  author={B W McGuire and Marie-Claire Orgebin-Crist and Frank Chytil},
  journal={Endocrinology},
  year={1981},
  volume={108 2},
  pages={658-67}
}
An interstitial target site in the rat testis for vitamin A has been detected using radioactively labeled rretinol-binding protein (RBP) in autoradiography at the light microscope level. RBP purified from rat serum was iodinated by the lactoperoxidase procedure and was also complexes with [3H]-retinol. The radioactively labeled RBP was administered to rat testis tissue by a direct intratesticular injection, an iv injection, and through an in vitro incubation of decapsulated tissue in medium… CONTINUE READING