Autoradiographic localisation of3H-apomorphine binding sites in rat brain

The best experimental conditions for a selective binding of3H-apomorphine to dopamine receptors on cryostat sections were first selected by liquid scintillation quantification of the bound radioactivity. In the corpus striatum, a specific binding occurred with a half-maximal saturation concentration of about 1 nM and a maximal capacity of 180 fmol/mg of… CONTINUE READING