Autophagy in tobacco BY-2 cells cultured under sucrose starvation conditions: isolation of the autolysosome and its characterization.

@article{Takatsuka2011AutophagyIT,
  title={Autophagy in tobacco BY-2 cells cultured under sucrose starvation conditions: isolation of the autolysosome and its characterization.},
  author={Chihiro Takatsuka and Yuko Inoue and Tomoya Higuchi and Stefan Hillmer and David G Robinson and Yuji Moriyasu},
  journal={Plant & cell physiology},
  year={2011},
  volume={52 12},
  pages={2074-87}
}
Tobacco culture cells carry out a large-scale degradation of intracellular proteins in order to survive under sucrose starvation conditions. We have previously suggested that this bulk degradation of cellular proteins is performed by autophagy, where autolysosomes formed de novo act as the major lytic compartments. The digestion process in autolysosomes can be retarded by addition of the cysteine protease inhibitor E-64c to the culture medium, resulting in the accumulation of autolysosomes. In… CONTINUE READING

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