Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery

@inproceedings{Roberts2013AutophagyAF,
  title={Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery},
  author={Rebecca Roberts and Wafa’ T. Al-Jamal and Matthew C Whelband and Paul Thomas and Matthew P. Jefferson and Jeroen Van den Bossche and Penny P. Powell and Kostas Kostarelos and Thomas M. Wileman},
  booktitle={Autophagy},
  year={2013}
}
Cationic liposome (lipoplex) and polymer (polyplex)-based vectors have been developed for nonviral gene delivery. These vectors bind DNA and enter cells via endosomes, but intracellular transfer of DNA to the nucleus is inefficient. Here we show that lipoplex and polyplex vectors enter cells in endosomes, activate autophagy and generate tubulovesicular autophagosomes. Activation of autophagy was dependent on ATG5, resulting in lipidation of LC3, but did not require the PtdIns 3-kinase activity… CONTINUE READING